[FILM-Users 00362] TOMORROW: FILM club Friday 31 May 1pm: Extracting complex information from images using Definiens Developer XD

Martin Spitaler m.spitaler at imperial.ac.uk
Thu May 30 10:17:48 BST 2013


Dear microscopists,

   in next week's FILM Club I will give an overview about the 
application of Definiens Developer XD for the analysis of complex 
microscopy data in the facility.

   Definiens Developer XD is a powerful software tool to extract complex 
information from unstructured image data, way beyond the usual
thresholding and filtering approaches biologists like to use. It gives 
biologists easy access to a large range of advanced image analysis 
tools, which allows to analyse images in a similar way to visual 
inspection, handling challenging problems like low contrast, label-free 
brightfield images, high noise and background, object tracking, etc. 
Tools like edge detection or various noise filters help dealing with 
limitations of image acquisition, while the problems of false positives 
/ negatives through strict thresholds can be overcome through fuzzy 
logics. Once objects are identified, a few clicks produce a wealth of 
results like compactness, elliptic fit, border index, position, 
orientation, relative distances and much more.Good imaging,

   In this presentation, I will give a short overview how these tools 
are used for the analysis of real data produced in the facility, where 
we have processed up to 30,000 images in one experiment.

   And please note that in September we are going to organise a 
Definiens Developer XD training course (and an Advanced course a bit 
later, probably in November), so if you have challenging microscopy data 
you need to analyse, this FILM Club should give you an idea whether this 
is your solution.


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FILM Club Friday 31 May, 1pm, SAFB-G120, South Kensington campus. For 
those who haven't been before, just walk into the Fleming building, and 
after the main glass entrance walk straight up the broad stairs, room 
G120 will be on your right-hand side.

http://www3.imperial.ac.uk/imagingfacility/news#filmclub

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Martin

http://www3.imperial.ac.uk/imagingfacility/equipment/licence_definiens
http://developer.definiens.com/algorithms.html

-- 
------------------------------------------------------------------------
*Martin Spitaler, PhD*

*FILM - Facility for Imaging by Light Microscopy*
- Facility Manager -
Sir Alexander Fleming Building, desk 401
Imperial College London / South Kensington
Exhibition Road
London SW7 2AZ
UK

Tel. +44-(0)20-759-42023
E-mail m.spitaler at imperial.ac.uk <mailto:m.spitaler at imperial.ac.uk>
Website: http://imperial.ac.uk/imagingfacility

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