[FILM-Users 00385] TODAY: FILM Microscopy Day 2013 - 28 June 2013 with plenary talk Spender Shorte on "FUEL Bioluminescence"

[Martin Spitaler]

Dear microscopists,

   here the latest update on today's FILM Microscopy Day, including 
*Spencer Shorte's invited talk: "FUEL for thought: a new method for 
detection of spatial proximity on mesoscopic scales in vitro and in vivo 
using luminescence excitation"* (4.30pm, G34, see abstract below) and 
the list of short presentations during the Microscopy Match Maker.

See you there,

Martin


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*FILM MICROSCOPY DAY 2013**
**Friday 28 June 2013**
**G34**
***

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PROGRAMME:
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      Morning session

*10.00-11.00         Basics of microscopy (Martin Spitaler, FILM)*
magnification, properties of light, resolution, transmitted light 
contrast (PH, DIC, darkfield), epifluorescence, confocal microscopy

*11.00-11.45         Live imaging techniques (Mark Scott, FILM) *
Multiphoton, in vivo imaging, second harmonic generation (SHG), 
photobleaching, phototoxicity

*11.45-12.00         coffee & tea break*


      MICROSCOPY MATCH-MAKER

*12.000-13.00        Microscopy Match-Maker:*
FILM users present their research performed in FILM through 3 min 
snapshot presentations

*Alejandra Garcia-Cattaneo*

	

Ajuba is a key component for adherens junction maintenance

*Ciro Chiappini*

	

Combining Confocal and Electron Microscopy to investigate the 
cell-nanoneedle interface

*Edwin Hawkins*

	

*Jill Johnson*

	

3D imaging of airway remodelling in asthmatic mice

*Kevin Woollard*

	

Study of in-vivo monocyte and macrophage biology by intravital microscopy

*Kim Jonas*

	

The Use of simultaneous dual colour PALM for imaging of receptor complexes

*Reema Khorshed*

	

Computational analysis of in vivo images

*Ryan Robinson*

	

Ca2+ Imaging of Guinea Pig Ganglia Neurons -- Investigating the effects 
of Diesel Exhaust Particles (DEP)

*Sean Warren*

	

FLIMFit: Rapid Global Analysis of FLIM Microscopy Data

*Sophie Schobesberger*

	

*Sowmya Purushothaman*

	

*William Jackson*

	

Tracking blood monocyte motility in vitro


*13.00-14.00         Sandwich lunch*


      Afternoon session

*14.00-14.45         A walk through the zoo: Molecular  imaging tools 
(Martin Spitaler, FILM)*
FRAP, FLIP, FLIM, FRET, FCS, FUEL, BRET and more

*14.45-15.30         Super-resolution microscopy (Steve Rothery, FILM) *
TIRF, PALM, STORM

*15.30-15.45         coffee & tea break*

*15.45-16.30         Understanding images (Luis Pizarro, Computing) *
basics of image data analysis, like noise removal, segmentation, 
tracking, registration


      PLENARY LECTURE

*16.30-17.30         Plenary talk:
                                 Spencer Shorte (Institute Pasteur, Paris):
                                 "FUEL for thought: a new method for 
detection of spatial proximity
                                   on mesoscopic scales in vitro and in 
vivo using luminescence excitation"*

Bioluminescence imaging is a powerful technique facilitating a broad 
range of applications from cell-based assay to in vivo analysis in 
living, intact organisms. The current state of the art uses 
Bioluminescence Resonance Energy Transfer (BRET) to improve the 
sensitivity of bioluminescence by red-shifting blue photons, and 
providing a measure of molecular co-localization at distances of up to 
10nm. However, BRET detection methods may overlook long-distance, 
radiating energy excitation-emission effects that are significant in the 
bioluminescent detection regime (1). Fluorescence by Unbound Excitation 
from Luminescence (FUEL) describes this radiating luminescence effect 
that excites fluorophores by epifluorescence at distances far beyond 
10nm, many microns, or even millimeters away in a manner completely 
distinct from BRET. Further, we show that detection of FUEL per se is 
sufficient to provide a detection of long-distance proximity in, and 
beyond the microscopic range both in vitro and in vivo. By enabling 
detection of mesoscopic proximity between luminescent and fluorescent 
probes in the context of living biological tissues FUEL promises utility 
as a novel tool for high-content analysis in cell and animal models.

1. Dragavon J, Blazquez S, Rekiki A, Samson C, Theodorou I, Rogers KL, 
Tournebize R, Shorte SL. In vivo excitation of nanoparticles using 
luminescent bacteria. Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):8890-5.


The plenary lecture will be followed by refreshments


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*Martin Spitaler, PhD*

*FILM - Facility for Imaging by Light Microscopy*
- Facility Manager -
Sir Alexander Fleming Building, desk 401
Imperial College London / South Kensington
Exhibition Road
London SW7 2AZ
UK

Tel. +44-(0)20-759-42023
E-mail m.spitaler at imperial.ac.uk <mailto:m.spitaler at imperial.ac.uk>
Website: http://imperial.ac.uk/imagingfacility



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